A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
نویسندگان
چکیده
منابع مشابه
A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences.
Chemical synthesis of DNA sequences provides a powerful tool for modifying genes and for studying gene function, structure and expression. Here, we report a simple, high-fidelity and cost-effective PCR-based two-step DNA synthesis (PTDS) method for synthesis of long segments of DNA. The method involves two steps. (i) Synthesis of individual fragments of the DNA of interest: ten to twelve 60mer ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 1362-4962
DOI: 10.1093/nar/gnh094